July 19, 2024

Screening of a genome-reduced Corynebacterium glutamicum

Screening of a genome-reduced Corynebacterium glutamicum strain library for improved heterologous cutinase secretion

  • The construction of microbial platform organisms by means of genome reduction is an ongoing topic in biotechnology. In this study, we investigated whether the deletion of single or multiple gene clusters has a positive effect on the secretion of cutinase from Fusarium solani pisi in the industrial workhorse Corynebacterium glutamicum.
  • A total of 22 genome-reduced strain variants were compared applying two Sec signal peptides from Bacillus subtilis. High-throughput phenotyping using robotics-integrated microbioreactor technology with automated harvesting revealed distinct cutinase secretion performance for a specific combination of signal peptide and genomic deletions.
  • The biomass-specific cutinase yield for strain GRS41_51_NprE was increased by ~ 200%, although the growth rate was reduced by ~ 60%. Importantly, the causative deletions of genomic clusters cg2801-cg2828 and rrnC-cg3298 could not have been inferred a priori. Strikingly, bioreactor fed-batch cultivations at controlled growth rates resulted in a complete reversal of the screening results, with the cutinase yield for strain GRS41_51_NprE dropping by ~ 25% compared to the reference strain.
  • Thus, the choice of bioprocess conditions may turn a ‘high-performance’ strain from batch screening into a ‘low-performance’ strain in fed-batch cultivation. In conclusion, future studies are needed in order to understand metabolic adaptations of C. glutamicum to both genomic deletions and different bioprocess conditions.
musagenomics
musagenomics

Recombinant Human rhinovirus 1A Genome polyprotein, partial

CSB-YP326367HQA 6222 mg Ask for price

Recombinant Human rhinovirus 1A Genome polyprotein,partial

AP72911 each Ask for price
  • Yeast

Recombinant Human rhinovirus 1A Genome polyprotein, partial

CSB-EP326367HQA 11613 mg Ask for price

Recombinant Human rhinovirus 1A Genome polyprotein, partial

MBS1220686-002mgEColi 0.02mg(E-Coli)
EUR 375

Recombinant Human rhinovirus 1A Genome polyprotein, partial

MBS1220686-002mgYeast 0.02mg(Yeast)
EUR 275

Recombinant Human rhinovirus 1A Genome polyprotein, partial

MBS1220686-01mgEColi 0.1mg(E-Coli)
EUR 640

Recombinant Human rhinovirus 1A Genome polyprotein, partial

MBS1220686-01mgYeast 0.1mg(Yeast)
EUR 450

Recombinant Human rhinovirus 1A Genome polyprotein, partial

MBS1220686-05mgYeast 0.5mg(Yeast)
EUR 1065

Recombinant Human rhinovirus 1A Genome polyprotein,partial

RPC25135-100ug 100ug
EUR 573.1

Recombinant Human rhinovirus 1A Genome polyprotein,partial

RPC25135-20ug 20ug
EUR 288.8

Recombinant Human rhinovirus 14 Genome polyprotein

MBS1158398-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1205

Recombinant Human rhinovirus 14 Genome polyprotein

MBS1158398-002mgEColi 0.02mg(E-Coli)
EUR 860

Recombinant Human rhinovirus 14 Genome polyprotein

MBS1158398-002mgYeast 0.02mg(Yeast)
EUR 995

Recombinant Human rhinovirus 14 Genome polyprotein

MBS1158398-01mgEColi 0.1mg(E-Coli)
EUR 1035

Recombinant Human rhinovirus 14 Genome polyprotein

MBS1158398-01mgYeast 0.1mg(Yeast)
EUR 1135

Recombinant Human rhinovirus 2 Genome polyprotein

MBS1030888-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1205

Recombinant Human rhinovirus 2 Genome polyprotein

MBS1030888-002mgEColi 0.02mg(E-Coli)
EUR 860

Recombinant Human rhinovirus 2 Genome polyprotein

MBS1030888-002mgYeast 0.02mg(Yeast)
EUR 995

Recombinant Human rhinovirus 2 Genome polyprotein

MBS1030888-01mgEColi 0.1mg(E-Coli)
EUR 1030

Recombinant Human rhinovirus 2 Genome polyprotein

MBS1030888-01mgYeast 0.1mg(Yeast)
EUR 1135

Recombinant Human rhinovirus 1B Genome polyprotein

MBS1154102-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1205

Recombinant Human rhinovirus 1B Genome polyprotein

MBS1154102-002mgEColi 0.02mg(E-Coli)
EUR 855

Recombinant Human rhinovirus 1B Genome polyprotein

MBS1154102-002mgYeast 0.02mg(Yeast)
EUR 995

Recombinant Human rhinovirus 1B Genome polyprotein

MBS1154102-01mgEColi 0.1mg(E-Coli)
EUR 1030

Recombinant Human rhinovirus 1B Genome polyprotein

MBS1154102-01mgYeast 0.1mg(Yeast)
EUR 1130

Recombinant Human rhinovirus 3 Genome polyprotein

MBS1326793-005mgBaculovirus 0.05mg(Baculovirus)
EUR 1125

Recombinant Human rhinovirus 3 Genome polyprotein

MBS1326793-005mgEColi 0.05mg(E-Coli)
EUR 860

Recombinant Human rhinovirus 3 Genome polyprotein

MBS1326793-005mgYeast 0.05mg(Yeast)
EUR 995

Recombinant Human rhinovirus 3 Genome polyprotein

MBS1326793-02mgEColi 0.2mg(E-Coli)
EUR 1120

Recombinant Human rhinovirus 3 Genome polyprotein

MBS1326793-05mgEColi 0.5mg(E-Coli)
EUR 1225

Recombinant Human rhinovirus 16 Genome polyprotein

MBS1325019-005mgBaculovirus 0.05mg(Baculovirus)
EUR 1120

Recombinant Human rhinovirus 16 Genome polyprotein

MBS1325019-005mgEColi 0.05mg(E-Coli)
EUR 860

Recombinant Human rhinovirus 16 Genome polyprotein

MBS1325019-005mgYeast 0.05mg(Yeast)
EUR 995

Recombinant Human rhinovirus 16 Genome polyprotein

MBS1325019-02mgEColi 0.2mg(E-Coli)
EUR 1120

Recombinant Human rhinovirus 16 Genome polyprotein

MBS1325019-05mgEColi 0.5mg(E-Coli)
EUR 1225

Recombinant Human Rhinovirus 16 VP3 (a.a. 331-562) [His]

DAG-WT1151 1 mg
EUR 2000
  • E. coli
Description: Recombinant

Recombinant Human Rhinovirus 16 VP4 (a.a. 2-69) [His]

DAG-WT1152 1 mg
EUR 2000
  • E. coli
Description: Recombinant

Human rhinovirus 1A Genome polyprotein

1-CSB-YP326367HQA
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  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 34.6 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Human rhinovirus 1A Genome polyprotein,partial expressed in Yeast

Recombinant Human rhinovirus 1B Genome polyprotein, partial

CSB-EP320160HQB 10401 mg Ask for price

CD54, Recombinant, Human (ICAM-1, Intercellular Adhesion Molecule1, Human rhinovirus receptor, Precursor, BB2, Major group rhinovirus receptor, P3.58)

MBS637697-001mg 0.01mg
EUR 555

CD54, Recombinant, Human (ICAM-1, Intercellular Adhesion Molecule1, Human rhinovirus receptor, Precursor, BB2, Major group rhinovirus receptor, P3.58)

MBS637697-5x001mg 5x0.01mg
EUR 2340

Recombinant Human rhinovirus A serotype 89 Genome polyprotein

MBS9422687-002mg 0.02mg
EUR 320

Recombinant Human rhinovirus A serotype 89 Genome polyprotein

MBS9422687-01mg 0.1mg
EUR 540

Recombinant Human rhinovirus A serotype 89 Genome polyprotein

MBS9422687-05mg 0.5mg
EUR 1360

Recombinant Human rhinovirus A serotype 89 Genome polyprotein

MBS9422687-5x05mg 5x0.5mg
EUR 5965

Recombinant Human rhinovirus A serotype 89 Genome polyprotein VP1

MBS1245807-002mgEColi 0.02mg(E-Coli)
EUR 255

Recombinant Human rhinovirus A serotype 89 Genome polyprotein VP1

MBS1245807-002mgYeast 0.02mg(Yeast)
EUR 270

Recombinant Human rhinovirus A serotype 89 Genome polyprotein VP1

MBS1245807-01mgEColi 0.1mg(E-Coli)
EUR 405

Recombinant Human rhinovirus A serotype 89 Genome polyprotein VP1

MBS1245807-01mgYeast 0.1mg(Yeast)
EUR 450

Recombinant Human rhinovirus A serotype 89 Genome polyprotein VP1

MBS1245807-05mgYeast 0.5mg(Yeast)
EUR 1065

Recombinant Human rhinovirus A serotype 89 Genome polyprotein VP1

MBS9421040-002mg 0.02mg
EUR 285

Recombinant Human rhinovirus A serotype 89 Genome polyprotein VP1

MBS9421040-01mg 0.1mg
EUR 470

Recombinant Human rhinovirus A serotype 89 Genome polyprotein VP1

MBS9421040-1mg 1mg
EUR 1785

Recombinant Human rhinovirus A serotype 89 Genome polyprotein VP1

MBS9421040-5x1mg 5x1mg
EUR 7885

Rhinovirus Type 1A Lysate

NAT41626-100 0.1
EUR 512.85
Description: Rhinovirus consists of a non-enveloped capsid containing a positive-sense single-stranded RNA.

Rhinovirus Type 1A Lysate

NAT41626-500 0.5
EUR 2181.53
Description: Rhinovirus consists of a non-enveloped capsid containing a positive-sense single-stranded RNA.

Recombinant Human rhinovirus A serotype 89 Genome polyprotein, partial

CSB-YP362073HQD 6387 mg Ask for price

Recombinant Human rhinovirus A serotype 89 Genome polyprotein,partial

AP71163 1mg
EUR 1978
  • E.coli

Recombinant Human rhinovirus A serotype 89 Genome polyprotein,partial

AP72984 each Ask for price
  • Yeast

Recombinant Human rhinovirus A serotype 89 Genome polyprotein,partial

AP77542 1mg
EUR 1978
  • E.coli

Recombinant Human rhinovirus A serotype 89 Genome polyprotein, partial

CSB-EP362073HQD 3389 mg Ask for price

Recombinant Human rhinovirus A serotype 89 Genome polyprotein, partial

CSB-EP362073HQDb0 3390 mg Ask for price

Recombinant Human rhinovirus A serotype 89 Genome polyprotein,partial

RPC22779-100ug 100ug
EUR 460.9

Recombinant Human rhinovirus A serotype 89 Genome polyprotein,partial

RPC22779-1mg 1mg
EUR 2237.8

Recombinant Human rhinovirus A serotype 89 Genome polyprotein,partial

RPC22779-20ug 20ug
EUR 258.7

NATtrol Human Rhinovirus Type 1A Stock(Qualitative) (1 mL)

NATHRV-STN 1 mL
EUR 1281

Recombinant Human rhinovirus A serotype 89 Genome polyprotein, partial, Biotinylated

CSB-EP362073HQD-B 7058 mg Ask for price

Recombinant Human rhinovirus A serotype 89 Genome polyprotein,partial,Biotinylated

RPC27677-100ug 100ug
EUR 614.3

Recombinant Human rhinovirus A serotype 89 Genome polyprotein,partial,Biotinylated

RPC27677-1mg 1mg
EUR 2373.6

Recombinant Human rhinovirus A serotype 89 Genome polyprotein,partial,Biotinylated

RPC27677-20ug 20ug
EUR 468

Recombinant Human rhinovirus A serotype 89 Genome polyprotein, partial, Biotinylated

MBS7137014-002mgEColi 0.02mg(E-Coli)
EUR 255

Recombinant Human rhinovirus A serotype 89 Genome polyprotein, partial, Biotinylated

MBS7137014-01mgEColi 0.1mg(E-Coli)
EUR 405

Recombinant Human rhinovirus A serotype 89 Genome polyprotein, partial, Biotinylated

MBS7137014-1mgEColi 1mg(E-Coli)
EUR 1425

Recombinant Human rhinovirus A serotype 89 Genome polyprotein, partial, Biotinylated

MBS7137014-5x1mgEColi 5x1mg(E-Coli)
EUR 6215

Human Rhinovirus(RV) ELISA Kit

SL3210Hu 96 Tests
EUR 468
  • 2-8°C

Human Rhinovirus,Rhv ELISA Kit

YLA2915HU-48T 48T Ask for price

Human Rhinovirus,Rhv ELISA Kit

YLA2915HU-96T 96T Ask for price

Human Rhinovirus,Rhv ELISA KIT

ED0457Hu 96 wells
EUR 458

Human Rhinovirus(Rhv)ELISA Kit

QY-E04146 96T
EUR 433.2

Human Rhinovirus IgG ELISA Kit

abx055879-100g 100 µg Ask for price

Human Rhinovirus IgG ELISA Kit

abx055879-10g 10 µg
EUR 493.75

Human Rhinovirus IgG ELISA Kit

abx055879-50g 50 µg Ask for price

Human Rhinovirus, Rhv ELISA Kit

MBS1610750-10x96StripWells 10x96-Strip-Wells
EUR 3460

Human Rhinovirus, Rhv ELISA Kit

MBS1610750-5x96StripWells 5x96-Strip-Wells
EUR 1750

Human Rhinovirus, Rhv ELISA Kit

MBS1610750-96StripWells 96-Strip-Wells
EUR 425

Genetic analysis of persistency indices of milk yield in Jersey crossbred cattle

The study reported in this Research Communication was conducted to estimate genetic parameters for different lactation persistency indices and their association with reproductive traits in Jersey crossbred cattle. Data on lactation traits (part lactation yields and 305-days milk yield) comprising all lactations as well as reproductive traits viz. calving interval, conception rate and gestation length of 378 Jersey crossbred cattle, maintained at National Dairy Research Institute, Kalyani, West Bengal, India, were collected over a period of 35 years (1982-2016).
Five lactation persistency indices of animals (P21, P31, P32, P4 and P5) were calculated using ratio method. A total of six different animal models, ignoring or including maternal genetic or permanent environmental effects, were fitted for all persistency traits. The best model was chosen after testing the improvement of the log-likelihood values.
The estimates of direct heritability were low in magnitude and ranged from 0.02 to 0.14 for the studied traits under the best fitted animal model. The permanent maternal environmental (c2) effects of different lactation persistency indices accounted for 2-9% of the total phenotypic variance in this study. Estimated genetic correlations of lactation persistency indices with all studied reproductive traits were low to moderate and negative (-0.11 to -0.68), except for calving interval.
Phenotypic correlations of lactation persistency measures with studied reproductive traits were low (0.01 to 0.15). The low heritability estimates of all the persistency indices indicate some limited scope of genetic improvement of lactation persistency of animals through selection under the prevailing management conditions.
Genome Wide Analysis of the Transcriptional Profiles in Different Regions of the Developing Rice Grains
Background: Rice is an important food source for humans worldwide. Because of its nutritional and agricultural significance, a number of studies addressed various aspects of rice grain development and grain filling. Nevertheless, the molecular processes underlying grain filling and development, and in particular the contributions of different grain tissues to these processes, are not understood.
Main text: Using RNA-sequencing, we profiled gene expression activity in grain tissues comprised of cross cells (CC), the nucellar epidermis (NE), ovular vascular trace (OVT), endosperm (EN) and the aleurone layer (AL). These tissues were dissected using laser capture microdissection (LCM) at three distinct grain development stages. The mRNA expression datasets offer comprehensive and new insights into the gene expression patterns in different rice grain tissues and their contributions to grain development.
Comparative analysis of the different tissues revealed their similar and/or unique functions, as well as the spatio-temporal regulation of common and tissue-specific genes. The expression patterns of genes encoding hormones and transporters indicate an important role of the OVT tissue in metabolite transport during grain development. Gene co-expression network prediction on OVT-specific genes identified several distinct and common development-specific transcription factors. Further analysis of enriched DNA sequence motifs proximal to OVT-specific genes revealed known and novel DNA sequence motifs relevant to rice grain development.
Conclusion: Together, the dataset of gene expression in rice grain tissues is a novel and useful resource for further work to dissect the molecular and metabolic processes during rice grain development.

Recombinant Human AITRL (AITRL Human)

MBS555501-0005mg 0.005mg
EUR 220

Recombinant Human AITRL (AITRL Human)

MBS555501-002mg 0.02mg
EUR 320

Recombinant Human AITRL (AITRL Human)

MBS555501-5x002mg 5x0.02mg
EUR 1300

TWEAK, recombinant / TNFSF12, recombinant (Human)

054-85 10 μg
EUR 261.36

Recombinant Human PGⅡ

E21-RA29-1 - Ask for price

Recombinant Human GC

MBS7616369-005mg 0.05mg
EUR 405

Recombinant Human GC

MBS7616369-02mg 0.2mg
EUR 760

Recombinant Human GC

MBS7616369-1mg 1mg
EUR 2175

Recombinant Human GC

MBS7616369-5x1mg 5x1mg
EUR 8410

Recombinant Human GH

MBS7608417-005mg 0.05mg
EUR 405

Recombinant Human GH

MBS7608417-02mg 0.2mg
EUR 760

Recombinant Human GH

MBS7608417-1mg 1mg
EUR 2175

Recombinant Human GH

MBS7608417-5x1mg 5x1mg
EUR 8410

Recombinant Human GS

MBS7608504-005mg 0.05mg
EUR 345

Recombinant Human GS

MBS7608504-02mg 0.2mg
EUR 635

Recombinant Human GS

MBS7608504-1mg 1mg
EUR 1800

Recombinant Human GS

MBS7608504-5x1mg 5x1mg
EUR 6955

Recombinant Human C6

MBS7608602-005mg 0.05mg
EUR 345

Recombinant Human C6

MBS7608602-02mg 0.2mg
EUR 635

Recombinant Human C6

MBS7608602-1mg 1mg
EUR 1800

Recombinant Human C6

MBS7608602-5x1mg 5x1mg
EUR 6955

Recombinant Human AR

MBS7612815-005mg 0.05mg
EUR 405

Recombinant Human AR

MBS7612815-02mg 0.2mg
EUR 760

Recombinant Human AR

MBS7612815-1mg 1mg
EUR 2175

Recombinant Human FH

MBS7613325-005mg 0.05mg
EUR 405

Recombinant Human FH

MBS7613325-02mg 0.2mg
EUR 760

Recombinant Human FH

MBS7613325-1mg 1mg
EUR 2175

Recombinant Human FH

MBS7613325-5x1mg 5x1mg
EUR 8410

Recombinant Human HP

MBS7614251-005mg 0.05mg
EUR 405

Recombinant Human HP

MBS7614251-02mg 0.2mg
EUR 760

Recombinant Human HP

MBS7614251-1mg 1mg
EUR 2175

Recombinant Human HP

MBS7614251-5x1mg 5x1mg
EUR 8410

Recombinant Human AR

MBS7612815-5x1mg 5x1mg
EUR 8410

Recombinant Human CP

MBS7613562-005mg 0.05mg
EUR 405

Recombinant Human CP

MBS7613562-02mg 0.2mg
EUR 760

Recombinant Human CP

MBS7613562-1mg 1mg
EUR 2175

Recombinant Human CP

MBS7613562-5x1mg 5x1mg
EUR 8410

Recombinant Human TH

MBS7613924-005mg 0.05mg
EUR 345

Recombinant Human TH

MBS7613924-02mg 0.2mg
EUR 635

Recombinant Human TH

MBS7613924-1mg 1mg
EUR 1800

Recombinant Human TH

MBS7613924-5x1mg 5x1mg
EUR 6955

Recombinant Human C5

MBS7609068-005mg 0.05mg
EUR 345

Recombinant Human C5

MBS7609068-02mg 0.2mg
EUR 635

Recombinant Human C5

MBS7609068-1mg 1mg
EUR 1800

Recombinant Human C5

MBS7609068-5x1mg 5x1mg
EUR 6955

Recombinant Human Rb

MBS7609212-005mg 0.05mg
EUR 470

Recombinant Human Rb

MBS7609212-02mg 0.2mg
EUR 885

Recombinant Human Rb

MBS7609212-1mg 1mg
EUR 2540

Recombinant Human Rb

MBS7609212-5x1mg 5x1mg
EUR 9820

Recombinant Human FH

MBS7609262-005mg 0.05mg
EUR 560

Recombinant Human FH

MBS7609262-02mg 0.2mg
EUR 1060

Recombinant Human FH

MBS7609262-1mg 1mg
EUR 3065

Recombinant Human FH

MBS7609262-5x1mg 5x1mg
EUR 11850

Recombinant Human C4

MBS7609327-005mg 0.05mg
EUR 405

Recombinant Human C4

MBS7609327-02mg 0.2mg
EUR 760

Recombinant Human C4

MBS7609327-1mg 1mg
EUR 2175

Recombinant Human C4

MBS7609327-5x1mg 5x1mg
EUR 8410

Recombinant Human C4

MBS7609328-005mg 0.05mg
EUR 405

Recombinant Human C4

MBS7609328-02mg 0.2mg
EUR 760

Recombinant Human C4

MBS7609328-1mg 1mg
EUR 2175

Recombinant Human C4

MBS7609328-5x1mg 5x1mg
EUR 8410

Recombinant Human GH

P1223-.1 10X10ug
EUR 240
Description: Peptides & Proteins|Recombinant Proteins

Recombinant Human GH

P1223-.5 50X10ug
EUR 538.4
Description: Peptides & Proteins|Recombinant Proteins

DR3 (human):Fc (human), (recombinant)

MBS566215-005mg 0.05mg
EUR 585

DR3 (human):Fc (human), (recombinant)

MBS566215-5x005mg 5x0.05mg
EUR 2585

Recombinant Human REC8

MBS7613173-005mg 0.05mg
EUR 405

Recombinant Human REC8

MBS7613173-02mg 0.2mg
EUR 760

Recombinant Human REC8

MBS7613173-1mg 1mg
EUR 2175

Recombinant Human REC8

MBS7613173-5x1mg 5x1mg
EUR 8410

Recombinant Human PRL

AP76222 1mg
EUR 2369
  • Human Cells

Recombinant Human PRL

CM10-10ug 10ug
EUR 169.2
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.

Recombinant Human PRL

CM10-1mg 1mg
EUR 2008.8
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.

Recombinant Human PRL

CM10-500ug 500ug
EUR 1423.2
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.

Recombinant Human PRL

CM10-50ug 50ug
EUR 363.6
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.

Recombinant Human BAD

7-04501 2µg Ask for price

Recombinant Human BAD

7-04502 5µg Ask for price

Recombinant Human BAD

7-04503 10µg Ask for price

Recombinant Human CD2

7-04717 2µg Ask for price

Recombinant Human CD2

7-04718 5µg Ask for price

Recombinant Human CD2

7-04719 10µg Ask for price

Recombinant Human CD9

7-04741 2µg Ask for price

Recombinant Human CD9

7-04742 5µg Ask for price

Recombinant Human CD9

7-04743 10µg Ask for price

Recombinant Human p53

7-05785 2µg Ask for price

Recombinant Human p53

7-05786 5µg Ask for price

Recombinant Human p53

7-05787 10µg Ask for price

Recombinant Human CSK

Z500098 10 µg
EUR 370
Description: CSK is a cytoplasmic tyrosine kinase that has been shown to downregulate the tyrosine kinase activity of the c-src through tyrosine phosphorylation of the c-src carboxy terminus . A yeast 2-hybrid system has been used to identify proteins associated with CSK. The Src homology-3 (SH3) domain of CSK associates with a proline-rich region of PEP, a protein-tyrosine phosphatase expressed in hemopoietic cells . This association is highly specific and it is speculated that PEP may be an effector and/or regulator of CSK in T cells and other hemopoietic cells.