by Genome transfer for the prevention of female infertility caused by maternal gene mutation
Poor oocyte quality is associated with early embryo developmental arrest and infertility. Maternal gene plays crucial roles in the regulation of oocyte maturation, and its mutation is a common cause of female infertility.However, how to improve oocyte quality and develop effective therapy for maternal gene mutation remains elusive. Here, we use Zar1 as an example to assess the feasibility of genome transfer to cure maternal gene mutation-caused female infertility.
We first discover that cytoplasmic deficiency primarily leads to Zar1-null embryo developmental arrest by disturbing maternal transcript degradation and minor zygotic genome activation (ZGA) during the maternal-zygotic transition. We next perform genome transfer at the oocyte (spindle transfer or polar body transfer) and zygote (early pronuclear transfer or late pronuclear transfer) stages to validate the feasibility of preventing Zar1 mutation-caused infertility.
We finally demonstrate that genome transfer either at the oocyte or at the early pronuclear stage can support normal preimplantation embryo development and produce live offspring. Moreover, those pups grow to adulthood and show normal fertility. Therefore, our findings provide an effective basis of therapies for the treatment of female infertility caused by maternal gene mutation
 Genome polyprotein Antibody |
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| 1-CSB-PA333180LA01HFD | Cusabio |
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Description: A polyclonal antibody against Genome polyprotein. Recognizes Genome polyprotein from Hepatitis C virus genotype 1a. This antibody is Unconjugated. Tested in the following application: ELISA |
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Genome polyprotein Antibody |
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| 1-CSB-PA362073LA01HQD | Cusabio |
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Description: A polyclonal antibody against Genome polyprotein. Recognizes Genome polyprotein from Human rhinovirus A serotype 89. This antibody is Unconjugated. Tested in the following application: ELISA |
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Genome polyprotein Antibody |
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| CSB-PA4643XA11GSV | Cusabio | 100ul / 50ul | EUR 299 |
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Description: Genome polyprotein |
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Genome polyprotein Antibody |
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| CSB-PA4644XA11GSW | Cusabio | 100ul / 50ul | EUR 299 |
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Description: Genome polyprotein |
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Genome polyprotein Antibody |
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| 1-CSB-PA714772LA01HYH | Cusabio |
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Description: A polyclonal antibody against Genome polyprotein. Recognizes Genome polyprotein from Human enterovirus 71. This antibody is Unconjugated. Tested in the following application: ELISA |
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Genome polyprotein Antibody |
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| abx300967-100g | Abbexa | 100 µg | EUR 362.5 |
Genome polyprotein Antibody |
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| abx300967-20g | Abbexa | 20 µg | EUR 162.5 |
Genome polyprotein Antibody |
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| abx300967-50g | Abbexa | 50 µg | EUR 250 |
 Genome Polyprotein Antibody |
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| abx109417-100l | Abbexa | 100 µl | EUR 162.5 |
Genome polyprotein Antibody |
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| MBS7147310-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Genome polyprotein Antibody |
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| MBS7149174-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Genome polyprotein Antibody |
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| MBS7152538-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Genome polyprotein Antibody |
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| MBS7152900-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Genome polyprotein Antibody |
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| MBS7153436-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Genome polyprotein Antibody |
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| MBS7153583-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Genome polyprotein Antibody |
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| MBS7150431-005mL | MyBiosource | 0.05mL | EUR 190 |
Genome polyprotein Antibody |
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| MBS7150431-01mL | MyBiosource | 0.1mL | EUR 270 |
Genome polyprotein Antibody |
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| MBS7150431-5x01mL | MyBiosource | 5x0.1mL | EUR 1205 |
Genome polyprotein Antibody |
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| MBS7165482-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Genome polyprotein Antibody |
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| MBS9004166-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Genome polyprotein Antibody |
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| MBS9000495-INQUIRE | MyBiosource | INQUIRE | Ask for price |
) Genome polyprotein Antibody (HRP) |
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| 20-abx109095 | Abbexa |
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Genome polyprotein Antibody (HRP) |
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| 20-abx300968 | Abbexa |
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Genome polyprotein Antibody (HRP) |
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| abx300968-100g | Abbexa | 100 µg | EUR 362.5 |
Genome polyprotein Antibody (HRP) |
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| abx300968-20g | Abbexa | 20 µg | EUR 162.5 |
Genome polyprotein Antibody (HRP) |
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| abx300968-50g | Abbexa | 50 µg | EUR 250 |
Genome polyprotein Antibody (HRP) |
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| abx109095-100l | Abbexa | 100 µl | EUR 162.5 |
) Genome polyprotein Antibody (FITC) |
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| 20-abx107678 | Abbexa |
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Genome polyprotein Antibody (FITC) |
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| 20-abx300969 | Abbexa |
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Genome polyprotein Antibody (FITC) |
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| abx107678-100g | Abbexa | 100 µg | EUR 362.5 |
Genome polyprotein Antibody (FITC) |
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| abx107678-20g | Abbexa | 20 µg | EUR 162.5 |
Genome polyprotein Antibody (FITC) |
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| abx107678-50g | Abbexa | 50 µg | EUR 250 |
Genome polyprotein Antibody (FITC) |
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| abx300969-100g | Abbexa | 100 µg | EUR 362.5 |
Genome polyprotein Antibody (FITC) |
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| abx300969-20g | Abbexa | 20 µg | EUR 162.5 |
Genome polyprotein Antibody (FITC) |
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| abx300969-50g | Abbexa | 50 µg | EUR 250 |
A novel fumarate, isosorbide di-(methyl fumarate) (IDMF), replicates astrocyte transcriptome responses to dimethyl fumarate (DMF) but specifically down-regulates genes linked to a reactive phenotype
Dimethyl fumarate (DMF) has emerged as a first-line treatment for the relapsing-remitting multiple sclerosis (RRMS) subtype. It is hypothesized that DMF has anti-inflammatory and antioxidant effects although mechanisms are not fully understood. This study used RNA-seq to profile gene expression responses to DMF in cultured astrocytes. Responses were compared with those of isosorbide di-(methyl fumarate) (IDMF), a newly designed fumarate that may partially replicate DMF activity with fewer adverse effects.
Both compounds altered the expression of MS-associated genes, including those near MS susceptibility loci and genes dysregulated in MS patient astrocytes. The shared DMF/IDMF transcriptome response involved altered expression of antioxidant genes (e.g., HMOX1) and genes linked to extracellular matrix integrity (TIMP3, MMP9) and migration of pro-inflammatory cells into CNS (CCL2). IDMF-specific transcriptome responses included down-regulation of mitotic genes associated with a proliferative reactive astrocyte phenotype (ICAM1) and repression of genes encoding NF-kappaB subunits (NFKB2, RELA, RELB) and NF-kappaB targets (NCAPG, CXCL1, OAS3).
Overall, these results identify astrocyte-centered mechanisms that may contribute to the established efficacy of DMF as an RRMS treatment. Furthermore, our findings support a rationale for further studies of IDMF as a novel fumarate, which may have unique suppressive effects on astrocyte reactivity and glial scar formation. [200 words].
Changes in peripheral blood oxidative stress markers and hepatic gene expression related to oxidative stress in Holstein cows with and without subacute ruminal acidosis during the periparturient period
- We investigated changes in peripheral blood metabolites, oxidative stress markers (malondialdehyde, potential antioxidant capacity, and glutathione peroxidase [GPX]), and hepatic gene expression related to oxidative stress in Holstein cows with and without subacute ruminal acidosis (SARA) during the periparturient period.
- Eighteen multiparous Holstein cows were categorized into SARA (n=9) or non-SARA (n=9) groups depending on whether they developed SARA; reticulo-ruminal pH was <5.6 for more than 3 hr per day, during the 2 weeks after parturition. Blood and liver tissue samples were collected 3 weeks prepartum and 2 and 6 weeks postpartum, with an additional blood sample collected 0 and 4 weeks postpartum. Blood aspartate transaminase (AST) and nonesterified fatty acid (NEFA) increased significantly (P<0.05) after parturition in both groups.
- GPX activity decreased gradually after parturition in the SARA group. In the SARA group, gene expression of GPX 1 and microsomal glutathione S-transferase 3 (MGST3) decreased significantly (P<0.05), and expression of metallothionein 2A increased significantly (P<0.05) after parturition in the SARA group. Superoxide dismutase 1 and MGST3 decreased significantly (P<0.05) 2 weeks postpartum in the non-SARA group. Gene expression related to oxidative stress was negatively correlated with AST, NEFA and total ketone body levels.
- Therefore, the hepatic gene expression related to oxidative stress might change associated with a negative energy balance, and might relate the high oxidative stress in the SARA group during periparturient period.
Genome polyprotein Antibody |
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| CSB-PA4643XA11GSV | Cusabio | 100ul / 50ul | EUR 299 |
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Description: Genome polyprotein |
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Genome polyprotein Antibody |
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| CSB-PA4644XA11GSW | Cusabio | 100ul / 50ul | EUR 299 |
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Description: Genome polyprotein |
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Genome polyprotein Antibody |
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| 1-CSB-PA714772LA01HYH | Cusabio |
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Description: A polyclonal antibody against Genome polyprotein. Recognizes Genome polyprotein from Human enterovirus 71. This antibody is Unconjugated. Tested in the following application: ELISA |
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Genome polyprotein Antibody |
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| abx300967-100g | Abbexa | 100 µg | EUR 362.5 |
Genome polyprotein Antibody |
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| abx300967-20g | Abbexa | 20 µg | EUR 162.5 |
Genome polyprotein Antibody |
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| abx300967-50g | Abbexa | 50 µg | EUR 250 |
Genome Polyprotein Antibody |
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| abx109417-100l | Abbexa | 100 µl | EUR 162.5 |
Genome polyprotein Antibody |
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| MBS7147310-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Genome polyprotein Antibody |
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| MBS7149174-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Genome polyprotein Antibody |
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| MBS7152538-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Genome polyprotein Antibody |
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| MBS7152900-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Genome polyprotein Antibody |
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| MBS7153436-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Genome polyprotein Antibody |
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| MBS7153583-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Genome polyprotein Antibody |
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| MBS7150431-005mL | MyBiosource | 0.05mL | EUR 190 |
Genome polyprotein Antibody |
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| MBS7150431-01mL | MyBiosource | 0.1mL | EUR 270 |
Genome polyprotein Antibody |
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| MBS7150431-5x01mL | MyBiosource | 5x0.1mL | EUR 1205 |
Genome polyprotein Antibody |
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| MBS7165482-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Genome polyprotein Antibody |
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| MBS9004166-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Genome polyprotein Antibody |
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| MBS9000495-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Genome polyprotein Antibody (HRP) |
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| 20-abx109095 | Abbexa |
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Genome polyprotein Antibody (HRP) |
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| 20-abx300968 | Abbexa |
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Genome polyprotein Antibody (HRP) |
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| abx300968-100g | Abbexa | 100 µg | EUR 362.5 |
Genome polyprotein Antibody (HRP) |
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| abx300968-20g | Abbexa | 20 µg | EUR 162.5 |
Genome polyprotein Antibody (HRP) |
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| abx300968-50g | Abbexa | 50 µg | EUR 250 |
Genome polyprotein Antibody (HRP) |
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| abx109095-100l | Abbexa | 100 µl | EUR 162.5 |
