October 24, 2020

The Drosophila CG1674 gene encodes a Synaptopodin-like

The Drosophila CG1674 gene encodes a Synaptopodin-like 2 related protein that localizes to the Z-disc and is required for normal flight muscle development and function

Background: To identify novel myofibrillar components of the Drosophila flight muscles, we carried out a proteomic analysis of chemically demembranated flight muscle myofibrils, and characterized the knockdown phenotype of a novel gene identified in the screen, CG1674.
Results: The CG1674 protein has some similarity to vertebrate synaptopodin 2-like, and when expressed as a FLAG-tagged fusion protein, it was localized during development to the Z-disc and cytoplasm. Knockdown of CG1674 expression affected the function of multiple muscle types, and defective flight in adults was accompanied by large actin-rich structures in the flight muscles that resembled overgrown Z-discs. Localization of CG1674 to the Z-disc depended predominantly upon presence of the Z-disc component alpha-actinin, but also depended upon other Z-disc components, including Mask, Zasp52, and Sals. We also observed re-localization of FLAG-CG1674 to the nucleus in Alpha-actinin and sals knockdown animals.
Conclusions: These studies identify and characterize a previously unreported myofibrillar component of Drosophila muscle, that is necessary for proper myofibril assembly during development. This article is protected by copyright. All rights reserved.
musagenomics
musagenomics

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Accelerated cortical thinning and volume reduction over time in young people at high genetic risk for bipolar disorder

  • Background: Bipolar disorder (BD) is a familial psychiatric disorder associated with frontotemporal and subcortical brain abnormalities. It is unclear whether such abnormalities are present in relatives without BD, and little is known about structural brain trajectories in those at risk.
  • Method: Neuroimaging was conducted at baseline and at 2-year follow-up interval in 90 high-risk individuals with a first-degree BD relative (HR), and 56 participants with no family history of mental illness who could have non-BD diagnoses. All 146 subjects were aged 12-30 years at baseline. We examined longitudinal change in gray and white matter volume, cortical thickness, and surface area in the frontotemporal cortex and subcortical regions.
  • Results: Compared to controls, HR participants showed accelerated cortical thinning and volume reduction in right lateralised frontal regions, including the inferior frontal gyrus, lateral orbitofrontal cortex, frontal pole and rostral middle frontal gyrus. Independent of time, the HR group had greater cortical thickness in the left caudal anterior cingulate cortex, larger volume in the right medial orbitofrontal cortex and greater area of right accumbens, compared to controls. This pattern was evident even in those without the new onset of psychopathology during the inter-scan interval.
  • Conclusions: This study suggests that differences previously observed in BD are developing prior to the onset of the disorder. The pattern of pathological acceleration of cortical thinning is likely consistent with a disturbance of molecular mechanisms responsible for normal cortical thinning. We also demonstrate that neuroanatomical differences in HR individuals may be progressive in some regions and stable in others.
Challenges in gene-oriented approaches for pangenome content discovery
Given a group of genomes, represented as the sets of genes that belong to them, the discovery of the pangenomic content is based on the search of genetic homology among the genes for clustering them into families. Thus, pangenomic analyses investigate the membership of the families to the given genomes.
This approach is referred to as the gene-oriented approach in contrast to other definitions of the problem that takes into account different genomic features. In the past years, several tools have been developed to discover and analyse pangenomic contents. Because of the hardness of the problem, each tool applies a different strategy for discovering the pangenomic content. This results in a differentiation of the performance of each tool that depends on the composition of the input genomes. This review reports the main analysis instruments provided by the current state of the art tools for the discovery of pangenomic contents.
Moreover, unlike previous works, the presented study compares pangenomic tools from a methodological perspective, analysing the causes that lead a given methodology to outperform other tools. The analysis is performed by taking into account different bacterial populations, which are synthetically generated by changing evolutionary parameters.
The benchmarks used to compare the pangenomic tools, in addition to the computational pipeline developed for this purpose, are available at https://github.com/InfOmics/pangenes-review. Contact: V. Bonnici, R. Giugno Supplementary information: Supplementary data are available at Briefings in Bioinformatics online.

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  • Shipped within 5-10 working days.

Genome Polyprotein (HCV-Core) AssayLite Antibody (FITC Conjugate)

33487-05141 150 ug
EUR 428.00

Genome Polyprotein (HCV-Core) AssayLite Antibody (RPE Conjugate)

33487-05151 150 ug
EUR 428.00

Genome Polyprotein (HCV-Core) AssayLite Antibody (APC Conjugate)

33487-05161 150 ug
EUR 428.00

Genome Polyprotein (HCV-Core) AssayLite Antibody (PerCP Conjugate)

33487-05171 150 ug
EUR 471.00

Rhinovirus A serotype 89 Genome polyprotein Antibody (FITC)

20-abx107303
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 0
  • 1
  • 2
  • 3
  • 4
  • Shipped within 5-10 working days.

Rhinovirus A serotype 89 Genome polyprotein Antibody (HRP)

20-abx108723
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 0
  • 1
  • 2
  • 3
  • 4
  • Shipped within 5-10 working days.

Gag Polyprotein (GAG) Antibody (Biotin)

20-abx106336
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 0
  • 1
  • 2
  • 3
  • 4
  • Shipped within 5-10 working days.

Gag Polyprotein (GAG) Antibody (FITC)

20-abx107749
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 0
  • 1
  • 2
  • 3
  • 4
  • Shipped within 5-10 working days.

HIV-1 Gag-Pol polyprotein Antibody (Biotin)

20-abx106322
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 0
  • 1
  • 2
  • 3
  • 4
  • Shipped within 5-10 working days.

HIV-1 Gag-Pol polyprotein Antibody (FITC)

20-abx107735
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 0
  • 1
  • 2
  • 3
  • 4
  • Shipped within 5-10 working days.

HIV-1 Gag-Pol polyprotein Antibody (HRP)

20-abx109153
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 0
  • 1
  • 2
  • 3
  • 4
  • Shipped within 5-10 working days.

Moloney murine leukemia virus Gag polyprotein Antibody

20-abx110606
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 0
  • 1
  • 2
  • 3
  • 4
  • Shipped within 5-10 working days.

RecQ-Mediated Genome Instability Protein 2 (RMI2) Antibody

20-abx005864
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 0
  • 1
  • 2
  • 3
  • Shipped within 5-10 working days.

RecQ-Mediated Genome Instability Protein 1 (RMI1) Antibody

20-abx003807
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 0
  • 1
  • 2
  • 3
  • Shipped within 5-10 working days.

RecQ-Mediated Genome Instability Protein 1 (RMI1) Antibody

abx029125-400ul 400 ul
EUR 523.00
  • Shipped within 5-10 working days.

RecQ-Mediated Genome Instability Protein 1 (RMI1) Antibody

abx029125-80l 80 µl
EUR 286.00
  • Shipped within 5-10 working days.

RecQ-Mediated Genome Instability Protein 1 (RMI1) Antibody

20-abx115306
  • EUR 732.00
  • EUR 398.00
  • 0
  • 1
  • Shipped within 5-10 working days.

Moloney murine leukemia virus Gag polyprotein Antibody (HRP)

20-abx109167
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 0
  • 1
  • 2
  • 3
  • 4
  • Shipped within 5-10 working days.

HIV-1 Gag-Pol Polyprotein (gag-pol) Antibody

20-abx110593
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 0
  • 1
  • 2
  • 3
  • 4
  • Shipped within 5-10 working days.

CD11b Antibody Antibody

ABD2911 100 ug
EUR 438.00

Ly1 Antibody Reactive (LYAR) Antibody

20-abx008109
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 0
  • 1
  • 2
  • Shipped within 5-10 working days.

Anti-Glycolipid Antibody (AGA) Antibody

20-abx004855
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 0
  • 1
  • 2
  • 3
  • Shipped within 5-10 working days.

Ly1 Antibody Reactive (LYAR) Antibody

20-abx014333
  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • 0
  • 1
  • 2
  • 3
  • Shipped within 5-10 working days.

Ly1 Antibody Reactive (LYAR) Antibody

abx033330-400ul 400 ul
EUR 523.00
  • Shipped within 5-10 working days.

Ly1 Antibody Reactive (LYAR) Antibody

abx033330-80l 80 µl
EUR 286.00
  • Shipped within 5-10 working days.

Anti-Glycolipid Antibody (AGA) Antibody

abx036399-100ug 100 ug
EUR 391.00
  • Shipped within 5-10 working days.

FLI1 Antibody

33376-100ul 100ul
EUR 252.00

FLI1 Antibody

33376-50ul 50ul
EUR 187.00

GAD67 Antibody

33379-100ul 100ul
EUR 252.00

GAD67 Antibody

33379-50ul 50ul
EUR 187.00

Livin Antibody

3338-100
EUR 338.00

GANP Antibody

33383-100ul 100ul
EUR 252.00

GANP Antibody

33383-50ul 50ul
EUR 187.00

Gastrin Antibody

33384-100ul 100ul
EUR 252.00

Gastrin Antibody

33384-50ul 50ul
EUR 187.00

Glucagon Antibody

33385-100ul 100ul
EUR 252.00

Glucagon Antibody

33385-50ul 50ul
EUR 187.00

GluR4 Antibody

33387-100ul 100ul
EUR 252.00

GluR4 Antibody

33387-50ul 50ul
EUR 187.00

GluR5 Antibody

33388-100ul 100ul
EUR 252.00

GluR5 Antibody

33388-50ul 50ul
EUR 187.00

GluR6 Antibody

33389-100ul 100ul
EUR 252.00

GluR6 Antibody

33389-50ul 50ul
EUR 187.00

GluR7 Antibody

33390-100ul 100ul
EUR 252.00

GluR7 Antibody

33390-50ul 50ul
EUR 187.00

GluR8 Antibody

33391-100ul 100ul
EUR 252.00

GluR8 Antibody

33391-50ul 50ul
EUR 187.00

GLUT1 Antibody

33392-100ul 100ul
EUR 252.00

GLUT1 Antibody

33392-50ul 50ul
EUR 187.00

GLUT3 Antibody

33393-100ul 100ul
EUR 252.00

GLUT3 Antibody

33393-50ul 50ul
EUR 187.00

GRP78 Antibody

33395-100ul 100ul
EUR 252.00

GRP78 Antibody

33395-50ul 50ul
EUR 187.00

HDAC1 Antibody

33398-100ul 100ul
EUR 252.00

HDAC1 Antibody

33398-50ul 50ul
EUR 187.00

HDAC10 Antibody

33399-100ul 100ul
EUR 252.00

HDAC10 Antibody

33399-50ul 50ul
EUR 187.00

p53DINP1 Antibody

3340-100
EUR 327.00

p53DINP1 Antibody

3340-30T
EUR 146.00

HDAC3 Antibody

33400-100ul 100ul
EUR 252.00

HDAC3 Antibody

33400-50ul 50ul
EUR 187.00

HDAC5 Antibody

33401-100ul 100ul
EUR 252.00

HDAC5 Antibody

33401-50ul 50ul
EUR 187.00

HDAC7 Antibody

33402-100ul 100ul
EUR 252.00

HDAC7 Antibody

33402-50ul 50ul
EUR 187.00

HDAC9 Antibody

33403-100ul 100ul
EUR 252.00

HDAC9 Antibody

33403-50ul 50ul
EUR 187.00

HSP10 Antibody

33405-100ul 100ul
EUR 252.00

HSP10 Antibody

33405-50ul 50ul
EUR 187.00

HSP105 Antibody

33406-100ul 100ul
EUR 252.00

HSP105 Antibody

33406-50ul 50ul
EUR 187.00

HSP40 Antibody

33407-100ul 100ul
EUR 252.00

HSP40 Antibody

33407-50ul 50ul
EUR 187.00

HSP60 Antibody

33408-100ul 100ul
EUR 252.00

HSP60 Antibody

33408-50ul 50ul
EUR 187.00

Involucrin Antibody

33410-100ul 100ul
EUR 252.00

Involucrin Antibody

33410-50ul 50ul
EUR 187.00

JAB1 Antibody

33411-100ul 100ul
EUR 252.00

JAB1 Antibody

33411-50ul 50ul
EUR 187.00

JM4 Antibody

33412-100ul 100ul
EUR 252.00

JM4 Antibody

33412-50ul 50ul
EUR 187.00

Ki67 Antibody

33423-100ul 100ul
EUR 252.00

Ki67 Antibody

33423-50ul 50ul
EUR 187.00

iNOS Antibody

33424-100ul 100ul
EUR 252.00

iNOS Antibody

33424-50ul 50ul
EUR 187.00

Ku70 Antibody

33426-100ul 100ul
EUR 252.00

Ku70 Antibody

33426-50ul 50ul
EUR 187.00

Mammaglobin Antibody

33427-100ul 100ul
EUR 252.00

Mammaglobin Antibody

33427-50ul 50ul
EUR 187.00

MCL1 Antibody

33430-100ul 100ul
EUR 252.00

MCL1 Antibody

33430-50ul 50ul
EUR 187.00

MCM2 Antibody

33431-100ul 100ul
EUR 252.00

MCM2 Antibody

33431-50ul 50ul
EUR 187.00

MCM5 Antibody

33432-100ul 100ul
EUR 252.00

MCM5 Antibody

33432-50ul 50ul
EUR 187.00

MDM2 Antibody

33433-100ul 100ul
EUR 252.00

MDM2 Antibody

33433-50ul 50ul
EUR 187.00

NCK2 Antibody

33447-100ul 100ul
EUR 252.00

NCK2 Antibody

33447-50ul 50ul
EUR 187.00

NKX3.1 Antibody

33448-100ul 100ul
EUR 252.00

NKX3.1 Antibody

33448-50ul 50ul
EUR 187.00

NM23 Antibody

33449-100ul 100ul
EUR 252.00

NM23 Antibody

33449-50ul 50ul
EUR 187.00

NRF2 Antibody

33450-100ul 100ul
EUR 252.00

NRF2 Antibody

33450-50ul 50ul
EUR 187.00

NSE Antibody

33451-100ul 100ul
EUR 252.00

NSE Antibody

33451-50ul 50ul
EUR 187.00

OCT1 Antibody

33452-100ul 100ul
EUR 252.00

OCT1 Antibody

33452-50ul 50ul
EUR 187.00

OCT2 Antibody

33453-100ul 100ul
EUR 252.00

OCT2 Antibody

33453-50ul 50ul
EUR 187.00

OCT3 Antibody

33454-100ul 100ul
EUR 252.00

OCT3 Antibody

33454-50ul 50ul
EUR 187.00

Osteopontin Antibody

33455-100ul 100ul
EUR 252.00

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